Replicable expression vector in Mammalian Cells which comprises a promoter and Enhancer of the Gene Transcription of human immunoglobulin Kappa variable,Cell line that includes the Vector and Method for the production of recombinant human thrombopoietin polypeptides.

摘要

The present Invention refers to a Production System of Human thrombopoietin (htpo) Biologically Actibva by Mammalian Cells in Culture.Which were genetically modified by introduction of the Gene htpo under the form of a DNA Construct containing all the necessary elements for the expression of a htpo cmPleta.Furthermore, The Invention concerns the Potential Uses of the product obtained by this system (htpo) in Stimulating the Proliferation and differentiation of hematopoietic cellsS of megakaryocytic Line both in vitro and in vivo.