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Method for the detecting presence of catalytically active or catalytically inactive methyladenosine phosphatase
Method for the detecting presence of catalytically active or catalytically inactive methyladenosine phosphatase
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机译:检测具有催化活性或无催化活性的甲基腺苷磷酸酶的方法
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摘要
Catalytically active and inactive MTAse (methyladenosine phosphatase) are detected in mammalian cells by (1) treating cells suspected of being MTAse deficient with an oligonucleotide probe that hybridises specifically to an MTAse encoding nucleic acid and (2) detecting whether such nucleic acid is present. Also new are (1) isolated polynucleotides (I) encoding MTAse, (2) recombinant expression vectors contg. (I) or its fragments, (3) MTAse (or its fragments) expressed from these vectors or prepd. synthetically, (4) antibodies (Ab) raised against MTAse or its fragment.
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