HIGH LEVEL EXPRESSION, PURIFICATION AND REPEATED CREASING OF GROUP B(PORYNOWE?) PROTEINS OF OUTER MEMBRANE OF NEISSERIA MENINGITIDIS

摘要

The present invention relates, in general, to a method for the high level expression of the outer membrane protein meningococcal group B porin proteins and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein meningococcal group B porin proteins in E. coli wherein the meningococcal group B porin proteins yard fusion proteins thereof comprise more than 2% of the total protein expressed in E. coli. The invention also relates to a method of purification and refolding of the meningococcal group B porin proteins and fusion proteins thereof and to their use in vaccines.