New downstream regulatory element antagonist modulator gene and polypeptide that is a transcriptional repressor, for identifying antitumor agents

摘要

The DREAM (downstream regulatory element (DRE) antagonist modulator) gene (I) and polypeptide (II), in both native and mutated forms their use as specific transcriptional silencers of DRE sequences the use of (II) as a target for identifying new therapeutic compounds and the use as a regulator of CRE-dependent gene expression are new. Independent claims are also included for the following: (1) recombinant vectors containing (I) (2) host cells transformed with the vector of (a) (3) identifying therapeutic agents (A) able to block or activate (II) (4) use of embryonic cells of (2) for creating transgenic animals that have disorders associated with alterations in expression or activity of DREAM (5) a nucleic acid (III) comprising or containing a 2886 base pair (bp) sequence, representing the complementary sequence to DREAM mRNA (6) a recombinant vector containing (III) (7) host cells transfected with the vector of (6), with suppressed DREAM expression and (8) mono- or poly-clonal antibodies (Ab) that recognize (II).