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Diagnostic kit specifically for Leishmaniasis caused by 'Infantum Leishmaniasis' through the PCR-ELISA technique

机译:通过PCR-ELISA技术专门针对由'Infantum Leishmaniasis'引起的利什曼病的诊断试剂盒

摘要

Diagnostic kit specifically for leishmaniasis caused by 'Infantum Leishmaniasis' through the PCR-ELISA technique.'Infantum Leishmaniasis' is the only etiological agent from leishmaniasis in Spain and the majority of European Mediterranean countries. There are various techniques that are usually used to diagnose leishmaniasis these however give rise to a series of fundamental problems when diagnosing both human and canine leishmaniasis. The purpose of the 'Diagnostic kit specifically for leishmaniasis caused by 'Infantum Leishmaniasis' through the PCR-ELISA technique' is to improve sensitivity and specificity of leishmaniasis diagnostic techniques that are currently used, thus overcoming the existing problems. The kit is based on the usage, in a PCR, of chokes (9 and 83) that amplify a specific section of Infantum Leishmaniasis and an ELISA, of an internal oligonucleotid probe, marked with biotins, that hybridise with the amplified fragment in a specific way.
机译:通过PCR-ELISA技术专门针对由'Infantum Leishmaniasis'引起的利什曼病的诊断试剂盒'Infantum Leishmaniasis'是西班牙和大多数欧洲地中海国家唯一来自利什曼病的病原体。通常有多种技术可用于诊断利什曼病,然而,在诊断人和犬利什曼病时都会引起一系列基本问题。 “通过PCR-ELISA技术专门针对由'Infantum Leishmaniasis'引起的利什曼病的诊断试剂盒”的目的是提高当前使用的利什曼病诊断技术的敏感性和特异性,从而克服现有问题。该试剂盒基于在PCR中使用的扼流圈(9和83)的扩增,该扼流圈扩增了婴儿范氏利什曼病的特定部分,并通过ELISA标记了带有生物素的内部寡核苷酸探针,该探针与特异性片段中的扩增片段杂交道路。

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