RECOMBINANT EXPRESSION VECTORS AND PURIFICATION METHODS FOR THERMUS THERMOPHILUS DNA POLYMERASE

摘要

Recombinant DNA sequences encoding the DNA polymerase I activity of Thermusthermophilus can be used to constructrecombinant vectors and transformed host cells for production of the activity,T. thermophilus DNA polymerase I is an ~ 94 kDaprotein especially useful in the DNA amplification procedure known as thepolymerase chain reaction.