DYES DESIGNED FOR HIGH SENSITIVITY DETECTION OF DOUBLE-STRANDED DNA

摘要

Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a polycationic chain of at least two positivecharges. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleicacid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis andsolution at substantially higher sensitivities using substantially less dye.