COMPOSITIONS, METHODS AND SYSTEMS FOR THE DISCOVERY OF ENEDIYNE NATURAL PRODUCTS

摘要

Five protein families cooperate to form the warhead structure thatcharacterizesenediyne compounds, both chromoprotein enediynes and non-chromoproteinenediynes. The protein families include a polyketide synthase and thioesteraseproteinwhich form a polyketide synthase catalytic complex involved in warheadformation inenediynes. Genes encoding a member of each of the five protein families arefound inall enediyne biosynthetic loci. The genes and proteins may be used in geneticengineering applications to design new enediyne compounds and in methods toidentifynew enediyne biosynthetic loci.