METHODS OF LIGATION MEDIATED CHIMERAGENESIS UTILIZING POPULATIONS OF SCAFFOLD AND DONOR NUCLEIC ACIDS

摘要

The present invention is drawn to a method for forming at least one chimeric polynucleotide, methods for directed evolution, chimeric polynucleotides and libraries of chimeric polynucleotides. One method comprises contacting a first population of single-stranded oligonucleotides wherein the oligonucleotides share minimal complementarity with each other with a second population of oligonucleotides, under conditions wherein the oligonucleotides of the first and second populations hybridize to each other, forming at least one hybridized complex, comprising at least one polynucleotide from the first population hybridized to at least two oligonucleotides from the second population. Single-stranded regions are filled in using polymerase. The filled-in hybridized complex is treated such that the adjacent nucleic acids are ligated, forming at least one chimeric polynucleotide.