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Selective photoinduced flavin-dependent cleavage of RNA at G-U base pairs
Selective photoinduced flavin-dependent cleavage of RNA at G-U base pairs
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机译:G-U碱基对的选择性光诱导黄素依赖性RNA切割
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摘要
A method of and a kit for cleaving an RNA molecule containing at least one G-U wobble base pair at the at least one G-U wobble base pair thereof, comprising combining the RNA molecule with (a) a photosensitizing flavin derivative which is capable of performing a photooxidative reaction with the RNA molecule upon irradiation with light and (b) a divalent metal cation, or salt thereof, to produce a mixture; and thereafter cleaving the RNA molecule at the at least one G-U wobble base pair thereof by irradiating the mixture with light.
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