Use of Very Long Chain Fatty Acid Elongase for the identification of herbicides

摘要

Use of a polypeptide (I) with very long chain fatty acid elongase (VLCFAE) activity for identifying herbicides. Independent claims are also included for the following: (1) use of nucleic acid (II) that encodes (I) for identifying agents that either modulate (I) or alter expression of (II); (2) use of DNA constructs, or vectors, that contain a nucleic acid sequence (S1; 2782 base pair (bp) sequence defined in the specification), and a heterologous promoter for identifying herbicides; (3) similar use of host cells containing (I); (4) method for identifying compounds that bind to (I), including inhibitors and/or activators, or alter expression of (II); (5) use of the native regulatory sequences of (S1) to produce the constructs, vectors and host cells of (1)-(3), or in method (4); (6) modulators and herbicides identified by method (4); (7) use of (S1) for preparing transgenic plants; (8) transgenic plants (or their parts, protoplasts, tissues and replicative material) that contain (I) and show an altered intracellular content of (I); and (9) plants (or their parts, protoplasts, tissues and replicative material) in which the biological activity or expression of (I) has been altered.