/ PCR T/A cloning vector for cloning PCR product by single enzyme digestion and process for preparing thereof

摘要

The present invention relates to a PCR T / A cloning vector capable of cloning a polymerase chain reaction (PCR) product by a single enzyme cleavage, and a method for preparing the same. Specifically, both of the cloning vector can be achieved by a single cleavage of the restriction enzyme XcmI. A DNA fragment is prepared that forms a deoxythymine overhang at the 3'-end, and is used to readily bind to a deoxyadenine overhang located at both 3'-ends of the PCR product. And a PCR T / A cloning vector comprising a bidirectional multi-cloning site (MCS) around both 3'-terminal deoxythymine protrusions and a method for preparing the same. The DNA fragment of the present invention can be applied to an existing vector to prepare a PCR T / A cloning vector by a simple method in a short time, and the PCR T / A cloning vector thus prepared can be used for cloning PCR products as well as for re-eluting and re-closing. It facilitates cloning and can be used as a very useful tool in molecular biology research.