-3- Recombinant Microorganism Expressing Polyhydroxyalkanoate Biosynthesis Gene and Intracellular PHA Depolymerase Gene and Process for Preparing R-Hydroxycarboxylic Acid Using the Same

摘要

The present invention is poly-hydroxyalkanoic acids (polyhydroxyalkanoate, PHA), and recombinant plasmid containing the gene and using the same gene within the cell PHA-decomposing enzyme of the biosynthetic enzymes involved in (R) - hydroxy-carboxylic acid ((R) -hydroxycarboxylic acid a method of manufacturing a). The invention the LAL Stony ah oil Trojan wave intracellular PHA-decomposing enzyme (depolymerase) and Lal Stony ah oil Trojan to Ness radar's wave or alkaline PHA synthesis involved enzyme (biosynthesis enzyme) of (Alcaligenes latus) of (Ralstonia eutropha) with from this, by culturing a transformant with a recombinant plasmid expressing E. coli (R) - provides a method for producing a hydroxycarboxylic acid-optically pure (R) comprising the step of hydroxy to yield the hydroxy carboxylic acid. According to the present invention, the PHA synthesis and decomposition are the application of a continuous process possible due to take place at the same time, simplifies the disposal process of the cells were collected and cellular material and can expect a large effect of improving the productivity and economic efficiency. Further, by applying the in PHA-decomposing enzyme variety of PHA biosynthesis enzyme system and the cells from a microorganism capable of synthesizing PHA having a variety of different monomer units in the recombinant E. coli system, widely used in the production of a variety of 3-hydroxy-carboxylic acid It can be.