-3- Recombinant Microorganism Expressing Polyhydroxyalkanoate Biosynthesis Gene and Intracellular PHA Depolymerase Gene and Process for Preparing R-Hydroxycarboxylic Acid Using the Same

摘要

The present invention provides a recombinant plasmid comprising a gene of a polyhydroxyalkanoate (PHA) biosynthesis involved enzyme and a gene of PHA intracellular enzyme and a (R) -hydroxycarboxylic acid ((R) -hydroxycarboxylic acid using the same). It relates to a method of manufacturing). The present invention together with intracellular PHA depolymerase of Ralstonia eutropha and biosynthesis enzyme of PHA synthesis of Ralstonia eutropa or Alcaligenes latus It provides a method for producing an optically pure (R) -hydroxycarboxylic acid comprising the step of culturing E. coli transformed with the recombinant plasmid to express to obtain (R) -hydroxycarboxylic acid therefrom. According to the present invention, since PHA synthesis and decomposition occur at the same time, the present invention can be applied to a continuous process, and the cell recovery and disposal of cell materials can be simplified, so that a significant improvement in productivity and economy can be expected. In addition, by applying various kinds of PHA biosynthetic enzyme system and intracellular PHA degrading enzyme from microorganisms capable of synthesizing PHA having various monomer units in the recombinant E. coli system, it is widely used for the production of various 3-hydroxycarboxylic acids. Can be.