-1 A Novel Kluyveromyces delphensis IBN-H1 for Decomposing TMAH and Method for Waste-water Treatment using the Strain

摘要

PURPOSE: Provided are novel Kluyveromyces delphensis IBN-H1 decomposing tetramethyl ammonium hydroxide (TMAH) and a method for treating waste water using the same strain. TMAH is used in etching the surface of the silicon, and is accordingly contained in the waste water of a semiconductor plant. It is hardly decomposed and shows toxicity. This waste water containing TMAH is treated by the above novel strain eliminating 90% of TMAH therefrom. The process can be used in the industry as an environment-friendly waste water treatment. CONSTITUTION: The strain is prepared by: culturing strains in the waste water of semiconductor plants and culturing again the above strains in the medium containing TMAH; selecting 7 strains showing resistance to TMAH; and naming them as IBN H- 1-IBN H-7 and choosing IBN H-1, IBN H-4 and IBN H-7, which have excellent TMAH decomposability. They are identified as a novel strain belonging to Kluyveromyces delphensis, Bacillus cereus and Acinetobacter sp. by BIOLOG system, MIDI and partial base sequence of 16s RNA and named Kluyveromyces delphensis IBN H-1 (KCTC 0834BP), Bacillus cereus IBN H-4 (KCTC 0835BP) and Acinetobacter sp. IBN H-7 (KCTC 0836PB). The biological treatment of the waste water with TMAH is carried out by a batch culture, more preferably fed-batch culture or continuous culture.