MULTIPLEX PCR KIT AND OLIGONUCLEOTIDE FOR DETECTION AND IDENTIFICATION OF MYCOBACTERIA USING MULTIPLEX PCR

摘要

PURPOSE: Provided are a multiplex PCR method and a kit and an oligonucleotide for identifying Mycobacteria using the multiplex PCR, thereby determining whether a bacterium belongs to Mycobacterium sp. or not, and simultaneously detecting 4 kinds of Mycobacteria, M.tuberculosis, M.fortuitum, M.flavescens and M.avium. CONSTITUTION: The multiplex PCR method comprises: preparing a fixed primer by using at least one oligonucleotide of SEQ ID NO:1 having common nucelotide sequence of at least two target genes to be amplified; preparing specific primers by using at least two oligonucleotides of SEQ ID NO:2-6 having specific nucleotide sequences of the target genes; and reacting the fixed primer and the specific primers in a reactor to amplify at least two target genes.