5'- 4-82-9 5'- A microorganism Corynebacterium ammoniagenes NV4-82-9 being capable of producing 5'-xanthylic acid in higher yield and a producing method of 5'-xanthylic acid by using the same

摘要

PURPOSE: Provided are a microorganism, Corynebacterium ammoniagenes NV4-82-9, producing 5'-xanthylic acid in higher yield and having Norvaline resistance, and a production method of 5'-xanthylic acid by using the same. CONSTITUTION: Corynebacterium ammoniagenes NV4-82-9(KFCC-11248) produces 5'-xanthylic acid(XMP) and shows Norvaline resistance.Particularly, It is characterized by growing in the presence of an infinitesimal quantity of or 6g/l of Norvaline. 5'-xanthylic acid is produced by the steps of: firstly shacking culturing Corynebacterium ammoniagenes NV4-82-9(KFCC-11248) in a seed culture medium at 30 deg.C, pH 7.3 with 180 rpm for 24 hours; secondary shacking culturing the firstly cultured Corynebacterium ammoniagenes NV4-82-9(KFCC-11248) in a seed culture medium at 31 deg.C, pH 7.3 with 900 rpm for 24 hours to activate it; and shacking culturing the secondary cultured Corynebacterium ammoniagenes NV4-82-9(KFCC-11248) at 33 deg.C with 400 rpm for 90 hours in a fermentation medium, wherein if the content of sugar remaining in the culture solution is 1% or less, glucose can be added in the amount to reach total sugar content of 30%.