3--4- 3--4- 4- 34- 4- Natural Preservatives Using Peanut Shell Including 3-Methoxy-4-hydroxybenzoic Acid 3-Methoxy-4-hydroxycinnamic Acid 4-Hydroxybenzoic Acid 34-Dihydroxybenzoic Acid and 4-Hydroxycinnamic Acid

摘要

PURPOSE: Provided is a natural preservative using the skin of Arachis hypogaea containing natural antimicrobial and antioxidant active materials such as 3-methoxy-4-hydroxybenzoic acid, 3-methoxy-4-hydroxycinnamic acid, 4-hydroxybenzoic acid, 3,4-dihydroxybenzoic acid, 4-hydroxycinnamic acid, and etc. CONSTITUTION: The 3-methoxy-4-hydroxybenzoic acid, the 3-methoxy-4-hydroxycinnamic acid, the 4-hydroxybenzoic acid, the 3,4-dihydroxybenzoic acid, the 4-hydroxycinnamic acid are separated from the skin of Arachis hypogaea by a process comprising the steps of: extracting from the skin of Arachis hypogaea by methanol; distributing the extract by ethylacetate and a phosphoric acid buffer solution to obtain ethylacetate-soluble acidic fractions; separating the fractions by a silicagel absorption column chromatography, a sephadex LH-20 column chromatography, an octadecylsilane(ODS) column chromatography, and a HPLC.