The present invention relates to a method for producing a high yield of a variant strain Thermoactinomyces sp. M1 (KCTC 8864P) and a proteolytic enzyme using the same, more specifically, a glucose transport system is deleted to inhibit catabolic metabolism. (Cabolite repression) is characterized in that the method of producing a strain M1 strain of thermoactinomyces released and a high yield proteinase using the same, when applied to the fermentation process as the inhibition of catabolic metabolism is released It is possible to produce enzyme in high yield without inhibiting enzyme production by glucose in the medium, and also to increase the enzyme production by 2.1 times when producing protease using enzyme production medium. Enhancing fermentation yield during enzyme production has the effect of reducing the enzyme production cost.
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