POLYMER CONJUGATE OF RECOMBINANT HUMAN SUPEROXIDE DISMUTASE(RHSOD) AND PREPARATION METHOD THEREOF

摘要

PURPOSE: A polymer conjugate of recombinant human superoxide dismutase(rhSOD) and a preparation method thereof are provided, thereby increasing the half life of rhSOD, and maintaining the activity thereof. CONSTITUTION: A method for preparing the recombinant human superoxide dismutase(rhSOD) comprises the steps of: (1) oxidizing a hydroxy group of polyethylene glycol(PEG) or methoxy polyethylene glycol(mPEG) having the molecular weight of 4,000 to 8,000 to form a carboxyl derivative; (2) treating the carboxyl derivative with trimethylacetylchloride to activate the carboxyl derivative; and (3) bonding the activated derivative with a lysine residue of the recombinant human superoxide dismutase(rhSOD) via amide bonding.