METHOD FOR DETERMINING HELICOBACTER PYLORI-ASSOCIATED INTRAGASTRAL UREASE ACTIVITY

摘要

FIELD: medicine, medicinal microbiology and gastroenterology. SUBSTANCE: method deals with detecting the degree of bacterial seeding volume of gastric mucosa at helicobacter gastritis, gastroduodenitis and ulcerous disease. One should take a biopsy fragment of gastric mucosa and put it into commercial solution. Incubation mixture is subjected for exposure, PEC- colorimetry is conducted at 540 nm wave length to compare optic density with incubation time of biopsy fragment and the weight of biopsy fragment (units of optic density/mg biopsy material/min). At urease activity values ranged 11.5-4 U one should detect a low degree of bacterial seeding volume of gastric mucosa, at its value within 5-10 U a moderate degree is detected and in case its value ranges 11-19 U a high degree of H.pylori seeding volume is concluded on. The method is of high specificity, enables to conduct a semi-quantitative analysis of bacterial seeding volume of gastric mucosa. EFFECT: higher efficiency. 2 ex, 1 tbl