Separation of viable biological cells from their suspensions, comprises a process takes place in robust hydrocyclone with controlled maximum pressure

摘要

Introducing cells into the tangential inlet of the cylindrical top chamber of a hydrocyclone, following a helical path through the cone to a lower outlet at the base, where cell-enriched suspension is extracted, where an upper overflow outlet is arranged centrally in the cylindrical chamber to collect suspension depleted in cells, and the pressure drop between inlet and overflow outlet is 4 bar at most, is new. Preferred features: The pressure drop between inlet and overflow outlet is 2 bar. Cyclone dimensions are: length 2-12 mm; diameter 5-15 mm; cone taper 5 deg -20 deg ; inlet diagram. 0.5-4 mm; overflow outlet 1-5 mm; outlet unit introduction depth 1-6 mm; diameter 0.5-7 mm. A similar variant with slightly differing specification is proposed. Several similar or differing hydrocyclones are operated in parallel or serial arrangement, continuously or intermittently.