Apparatus, useful e.g. for spraying biologically compatible liquids, biologically compatible suspensions, biologically compatible cell suspensions, comprises hollow needle or cannula with hollow pin for separation of liquids or suspensions

摘要

Apparatus for spraying biologically compatible liquids, biologically compatible suspensions, and biologically compatible cell suspensions including stem cell suspensions, modified cell suspensions, cell suspensions for wound regeneration, cell suspensions of dermal cells, cell suspensions for epithelial cells, cell suspensions for dermal epithelium cells, which provides a continuous spray application over a period of 0.5-10 minutes, preferably 1-2 minutes, comprises a hollow needle or cannula having a hollow pin for the separation of liquids or suspensions. Apparatus for spraying biologically compatible liquids, biologically compatible suspensions, and biologically compatible cell suspensions including stem cell suspensions, modified cell suspensions, cell suspensions for wound regeneration, cell suspensions of dermal cells, cell suspensions for epithelial cells, cell suspensions for dermal epithelium cells, which provides a continuous spray application over a period of 0.5-10 minutes, preferably 1-2 minutes in a single spraying operation and/or multiple sprays, during which liquid or suspension drops of 10-500 mu m are generated, comprises a hollow needle or cannula having a hollow pin for the separation of liquids or suspensions. A biocompatible container is connected at the outlet and provides a point of the needle for liquid or suspension stream. The tip is introduced into a biocompatible tube, which provides a gas and/or air stream at the end of the tube and the tip of syringe is located at this end. The needle has an inner lumen of 25-34G, preferably 27-30G. The needle is inserted into the tube such that the needle tip ends within +- 3 mm, preferably +- 0.2 mm before the end of the tube. The needle tip within the tube runs at least 2-100 mm, preferably 4-20 mm parallel to the tube such that the needle point is created in the middle of the end of the tube lumen. The tube has an inner diameter of 1.5-0.2 mm, preferably 8-0.6 mm. The tube is bent at the point of the needle margin, where the lateral gas stream does not interfere with a radius in an angle of 1-170[deg] , preferably 80-100[deg] , and the needle margin is simple. The gas stream is an air stream, preferably a sterile filtered air stream. The liquid or suspension stream of 1-200 ml/minute, preferably 10-50 ml/minute, and a gas stream of 100-3000 ml/minute, preferably 200-1000 ml/minute, is provided during the gas stream. A compressor in a device or in a separate housing is connected through a tube to the device by the pump. The sterile filtering is carried out prior to entry into the needle with a sterile filter, during the liquid or suspension stream by a compressible container, preferably an injection syringe having a piston which is connected to an operating element including a pneumatic gas or hydraulic liquid membrane, or to a mechanical or electronic slide pressing the piston of the needle for producing the liquid or suspension stream. The device can be represented as a single, hand-held and optional battery-powered device, or as a device connected with at least two components with a hand-held spray device, and a cap member including electrical power supply which is connected by pneumatic, hydraulic or electric lines. The needle, tube or operation parameter configuration: produces a homogeneous and continuous spray, which passes through the stream of the liquid medium from the top and from the specific gaseous medium out of the tube; is less harmful to the cell suspension and used as a typical cell spray nozzle; and exhibits openings which are below and above the turbulence and configuration. All the hand-held parts of the apparatus are sterilized before use or the parts which contain gas and liquid or suspension are sterilized before using, or liquid or gas transport end parts are separately sterilized and assembled in sterile conditions before use. Independent claims are also included for: (1) a device is completely mechanically operated, and includes external compressed air or gas supply with pressure regulator and -control, and/or mechanically-operated syringe function, and/or manual syringe function; (2) producing an autologous cell suspension for a patient who requires a cell transplantation, comprising (a) preparing a cell suspension, and (b) applying the cell suspension to the affected area of the patient who requires cell transplantation to promote the uniform spraying of a cell suspension to a transplant area; (3) a cell suspension prepared by the above mentioned method and device; and (4) preparation and use of the cell suspension produced by the above mentioned method and device, where the cell suspension comprises in vitro expanded and/or non-cultured autologous mesenchymal adult progenitor cells and adult basal keratinocytes or progenitor cell-preparation.