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New DD1-a and DD1-b genes of maize and their regulators, useful for specific control of transgene expression in the transfer zone of grain during development

机译:玉米新的DD1-a和DD1-b基因及其调节剂,对发育过程中谷物转移区转基因表达的特异性控制有用

摘要

A novel nucleic acid (I) that is at least 80% identical with sequences (1) and (2), not reproduced, their fragments or the complements of (i). Independent claims are also included for the following: (a) a nucleic acid (Ia) containing at least 12 consecutive nucleotides (nt) from (I) or its complements; (b) a recombinant cloning and/or expression vector containing (I) or (Ia); (c) a host cell, or plant, transformed with (I), (Ia) or the vector of (b); (d) a method for preparing transgenic plants by regenerating plant cells of (c) or by transformation with Agrobacterium tumefaciens cells of (c); (e) transgenic plants produced by method (d) or their seeds; (f) polypeptides (II) encoded by (I); (g) antibodies (Ab) directed against (II); (h) detection of (II) by reaction with Ab; and (i) a kit for detecting (II) comprising Ab and optionally reagents for detecting an antigen/antibody complex.
机译:与未复制的序列(1)和(2),其片段或(i)的互补序列至少80%相同的新型核酸(I)。还包括以下方面的独立权利要求:(a)包含来自(I)或其补体的至少12个连续核苷酸(nt)的核酸(Ia); (b)含有(I)或(Ia)的重组克隆和/或表达载体; (c)用(I),(Ia)或(b)的载体转化的宿主细胞或植物; (d)通过再生(c)的植物细胞或通过用(c)的根癌农杆菌细胞转化来制备转基因植物的方法; (e)通过方法(d)产生的转基因植物或其种子; (f)由(I)编码的多肽(II); (g)针对(II)的抗体(Ab); (h)通过与Ab反应检测(II); (i)用于检测(II)的试剂盒,其包含Ab和任选地用于检测抗原/抗体复合物的试剂。

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