enhansa DNA base array and its application null of

摘要

PURPOSE: To obtain the subject new base sequence having a specific base sequence, bond to Aspergillus oryzae-derived α-glucosidase gene promoter domain, and capable of efficiently producing useful proteins, peptides using mould as host. ;CONSTITUTION: This new base sequence is a new enhancer DNA base sequence containing a sequence: -CGGNNATTTA-. This base sequence is incorporated into the promoter domain of an α-glucosidase gene derived from mould such as Aspergillus oryzae and binds a marker gene suitable for selecting the transformants of the mould as host, a terminator and a DNA domain replicable by Escherichia coli, and then incorporated into the host to enable useful proteins and peptides to be produced in high efficiency. This enhancer DNA base sequence is obtained by isolating the α-glucosidase gene promoter and by conducting a retrieval of this base sequence by using a gene-analyzing software.;COPYRIGHT: (C)1997,JPO