Culture method of denitrifying bacteria, immobilized denitrifying bacteria and denitrification method using the same

摘要

PURPOSE:To enable culture of high density in large quantity by a method wherein marine denitrification bacteria of specific genus Alkaligenes, wherein pH, temperature, and culture medium salt is in a specified optimum growth condition, are maintained in a predetermined condition and cultured until light absorbance becomes a predetermined value in a predetermined culture medium. CONSTITUTION:Marine denitrification bacteria of the genus Alkaligenes which has an optimum growth condition, wherein pH is 6-8, temperature 35 deg.C, and culture medium salt concentration is 0.85-3.4%, and has apparent metabolic pathway, wherein nitric acid is reduced directly to gaseous nitrogen, are selected. The bacteria are maintained in a condition similar to the optimum growth condition, such that salt concentration is 0.85-3.4%, pH 6-8, and temperature is 32-38 deg.C. And culture is continued until light absorbance of culture liquid becomes 2 or more at 610nm.