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BIOCHIP FOR DETECTING METHYLATION OF METHYLATION SITE IN GENOME DNA AND METHOD FOR DETECTING METHYLATION
BIOCHIP FOR DETECTING METHYLATION OF METHYLATION SITE IN GENOME DNA AND METHOD FOR DETECTING METHYLATION
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机译:用于检测基因组DNA中甲基化位点甲基化的生物芯片以及检测甲基化的方法
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摘要
PROBLEM TO BE SOLVED: To cyclopaedically analyze a methylation site in a genome DNA. ;SOLUTION: The genome DNA 700 is digested with a methylation-sensitive restriction enzyme SmaI among the methylation-sensitive restriction enzyme SmaI and methylation-insensitive restriction enzyme XmaI cleaving same recognition site and the genome DNA digested with SmaI is digested with XmaI. The DNA fragment 720 whose both ends are sites cleaved with XmaI is specifically amplified and the amplified DNA fragment is detected with a biochip in which the corresponding probe is fixed on a substrate.;COPYRIGHT: (C)2003,JPO
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