首页> 外国专利> METHOD FOR PREPARING SAMPLE FOR DETECTION USABLE FOR DETECTING METHYLATION OF DNA, METHOD FOR DETECTING METHYLATION OF DNA, SAMPLE-TREATING LIQUID AND KIT

METHOD FOR PREPARING SAMPLE FOR DETECTION USABLE FOR DETECTING METHYLATION OF DNA, METHOD FOR DETECTING METHYLATION OF DNA, SAMPLE-TREATING LIQUID AND KIT

机译:用于检测DNA的甲基化的检测用样品的制备方法,检测DNA的甲基化的方法,用于处理样品的液体和试剂盒

摘要

PROBLEM TO BE SOLVED: To provide a method for preparing a sample for detection by which an analyzing time can be shortened by simplifying a pretreatment method of a biosample usable for detecting the methylation of a DNA, and further to provide a method for detecting the methylation of the DNA, a sample-treating liquid and a kit.;SOLUTION: The method for preparing the sample for the detection usable for detecting the methylation of the DNA includes a step for mixing a biosample containing a cell with the sample-treating liquid containing a surfactant for solubilizing the cell, and a step for adding a converting agent for converting an unmethylated cytosine contained in the DNA to other bases.;COPYRIGHT: (C)2008,JPO&INPIT
机译:解决的问题:提供一种用于制备用于检测的样品的方法,通过简化可用于检测DNA的甲基化的生物样品的预处理方法,可以缩短分析时间,并且还提供一种用于检测甲基化的方法。解决方案:用于制备可用于检测DNA甲基化的检测样品的方法包括将含有细胞的生物样品与含有细胞的样品处理液混合的步骤。用于溶解细胞的表面活性剂和添加将DNA中包含的未甲基化胞嘧啶转化为其他碱基的转化剂的步骤。版权所有:(C)2008,JPO&INPIT

著录项

  • 公开/公告号JP2007259750A

    专利类型

  • 公开/公告日2007-10-11

    原文格式PDF

  • 申请/专利权人 SYSMEX CORP;

    申请/专利号JP20060088553

  • 发明设计人 ISHIHARA HIDEMIKI;KAJITA MASAHIRO;

    申请日2006-03-28

  • 分类号C12Q1/68;C12N15/09;G01N33/53;G01N33/566;

  • 国家 JP

  • 入库时间 2022-08-21 21:16:26

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