METHOD OF DNA ANALYSIS, DNA ANALYZER, AND PARTS FOR REACTION CHANNEL

摘要

PROBLEM TO BE SOLVED: To provide a method of DNA analysis capable of shortening analysis time of a base sequence and simplifying the analysis at a low cost and provide a DNA analyzer.;SOLUTION: This method of DNA analysis is to determine a DNA sequence in one base unit by adding 4 species of deoxynucleotide triphosphate (dNTP: dATP, dCTP, dGTP and dTTP) one by one to a DNA sample and successively detecting the elongation of a primer when the dNTP bonds the DNA sample. Each of the dNTP is marked with a fluorescent dye. Either one of the 4 fluorescent marked dNTP is added one by one to the DNA sample and reacted, with the DNA sample. Subsequently a reagent for decomposing the dNTP is added to the DNA sample to decompose the unreacted fluorescent marked dNTP out of the added fluorescent marked dNTP. After washing the DNA sample, the DNA sample is irradiated with lights. The fluorescent emitted from the fluorescent marked dNTP bonded to the DNA sample is detected to analyze whether the added dNTP bonds the DNA sample and the primer is elongated or not. The DNA analyzer is also provided.;COPYRIGHT: (C)2003,JPO