首页> 外国专利> PROMOTER DNA OF RICE CHLOROPLAST LIPOSOME PROTEIN L11 GENE

PROMOTER DNA OF RICE CHLOROPLAST LIPOSOME PROTEIN L11 GENE

机译:水稻叶绿体脂质体蛋白L11基因的启动子DNA

摘要

PROBLEM TO BE SOLVED: To obtain a new DNA encoding a promoter gene of a rice chloroplast liposome protein L11 (OsL11) gene.;SOLUTION: The DNA encoding the promoter of the rice OsL11 gene has a specific base sequence and 1,265 bp size. A DNA fragment DNA-P2 having a specified base sequence and 1,276 bp size is obtained by linking the DNA domain encoding the promoter gene, a DNA domain (cccaagt) of 6 bp for recognition of a restriction enzyme HindIII at the 5' side, and a DNA domain (agagc) of 5 bp for recognition of a restriction enzyme Xbal at the 3' side. A DNA having a specific bass sequence containing the DNA sequence of 1265 bp in the inside, and having other specified base sequence with an exon part to the DNA, and 1803 bp size is obtained as a DNA-P fragment. The DNA encoding the promoter of the OsL11 gene useful for creating a transformed plant as a promoter effective for carrying out the expression of a useful exogenous gene introduced into a plant body is provided.;COPYRIGHT: (C)2003,JPO
机译:解决的问题:获得编码水稻叶绿体脂质体蛋白L11(OsL11)基因启动子基因的新DNA。解决方案:编码水稻OsL11基因启动子的DNA具有特定的碱基序列和1,265 bp大小。通过连接编码启动子基因的DNA结构域,6 bp的DNA结构域(cccaagt)以识别5'侧限制性酶HindIII,并获得具有特定碱基序列和1,276 bp大小的DNA片段DNA-P2,并且5 bp的DNA结构域(agagc),用于识别3'侧的限制性酶Xbal。作为DNA-P片段,获得具有特定的低音序列的DNA,该特定的低音序列的内部含有1265bp的DNA序列,并且具有该DNA的外显子部分的其他特定碱基序列,并且具有1803bp的大小。提供了编码OsL11基因的启动子的DNA,该OsL11基因的启动子可用于有效地表达导入植物体内的有用的外源基因,而该启动子可有效地用于构建转化的植物。

著录项

  • 公开/公告号JP2003189877A

    专利类型

  • 公开/公告日2003-07-08

    原文格式PDF

  • 申请/专利权人 HOKKO CHEM IND CO LTD;

    申请/专利号JP20020265228

  • 发明设计人 TERAKAWA TERUHIKO;HASEGAWA HISAKAZU;

    申请日2002-09-11

  • 分类号C12N15/09;A01H1/00;C12N5/10;

  • 国家 JP

  • 入库时间 2022-08-22 00:14:10

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