SUCROSE PHOSPHATE SYNTHASE, METHOD FOR PRODUCING THE SAME, cDNA THEREOF AND UTILIZATION OF cDNA TO MODIFY EXPRESSION OF SPS IN PLANT CELL

摘要

PROBLEM TO BE SOLVED: To obtain a sucrose phosphate synthase (SPS) which can catalyze formation of sucrose phosphate and is obtained from corn. SOLUTION: The synthase protein containing an amino acid sequence having a specific sequence is obtained by (a) extraction from a part of corn plant by grinding, centrifugal separation and filtration, (b) increase of the ratio of extracted SPS in a solution, (c) purification of it with anion exchange high- performance chromatography, (d) further purification of active pool by using a high-performance chromatographic column and (e) if necessary, purification by a hybridoma and a monoclonal antibody.