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INSERTION FACTOR, TRANSPOSASE AND DNA SEQUENCE ENCODING TRANSPOSASE

机译:插入因子,转座酶和编码转座酶的DNA序列

摘要

PROBLEM TO BE SOLVED: To isolate an insertion factor derived from a chromosome of a bacterium of the genus Thermus, capable of carrying out transfer between bacteria of the genus Thermus and capable of being used for functional analysis of genes of bacteria belonging to the genus Thermus useful for production of proteins under the high-temperature environment and functional analysis of genes of bacteria belonging to the other genera and determine a base sequence of the insertion factor.;SOLUTION: An insertion factor IstaqTZ2 derived from Thermus aquaticus TZ2 strain is isolated and the base sequence thereof is determined. The insertion factor comprises a gene encoding a transposase in the sequence.;COPYRIGHT: (C)2003,JPO
机译:解决的问题:分离源自Thermus属细菌染色体的插入因子,其能够在Thermus属细菌之间进行转移,并能够用于对Thermus属细菌基因进行功能分析。可用于高温环境下的蛋白质生产以及对属于另一属的细菌的基因进行功能分析并确定插入因子的碱基序列。解决方案:分离出源自水生栖热菌TZ2菌株的插入因子IstaqTZ2,确定其碱基序列。插入因子包括编码该序列中的转座酶的基因。版权所有:(C)2003,JPO

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