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Telomeres of agrobacterium linear chromosome
Telomeres of agrobacterium linear chromosome
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机译:农杆菌线性染色体端粒
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摘要
Isolated telomeres from the linear chromosome of an Agrobacterium tumefaciens are obtainable from a restriction enzyme fragment at the end of said chromosome which is less than 4,000 nucleotide bases and comprises a segment of consecutive nucleotide bases having substantial identity to SEQ ID NO: 1 or SEQ ID NO: 2. The isolated telomeres are obtained by removing more or less of the segment from the larger restriction fragment. Pairs of isolated and distinct telomeres obtained from opposite ends of the linear chromosome are used for linear DNA constructs for use in producing transgenic plants by Agrobacterium tumefaciens transformation. Such constructs act as linear plasmids and comprise at least an origin of replication and terminal regions obtained from telomeres.
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机译:从根癌农杆菌的线性染色体分离的端粒可从所述染色体末端的限制酶片段获得,该片段小于4,000个核苷酸碱基,并且包含与SEQ ID NO:1具有实质同一性的连续核苷酸碱基的片段。 ID NO:1或SEQ ID NO:2。分离的端粒是通过从较大的限制性片段中去除或多或少的片段获得的。从线性染色体相对两端获得的成对的分离的和不同的端粒可用于线性DNA构建体,用于通过 Agrobacterium tumefaciens I>转化生产转基因植物。这样的构建体充当线性质粒,并且至少包含复制起点和从端粒获得的末端区域。
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