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Coupling of capillary electrophoresis (CE) with mass spectrometry (MS) with optimum separation
Coupling of capillary electrophoresis (CE) with mass spectrometry (MS) with optimum separation
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机译:毛细管电泳(CE)与质谱(MS)的耦合,具有最佳分离度
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摘要
The invention relates to methods used for mass spectrometric analysis of substances separated by capillary electrophoresis, in particular biopolymers such as proteins, proteoglycanes or other protein conjugates or their digest peptides. The invention consists in reducing the electrophoresis voltage upon appearance of the first analytically interesting substance, thereby maintaining the high separation power and gaining sensitivity. With direct coupling, e.g. by electrospray, the electrophoretic voltage may be directly controlled by the ion current measured by the mass spectrometer.
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