Highly sensitive trivalent copper chelate-luminol chemiluminescence system for capillary electrophoresis chiral separation and determination of ofloxacin enantiomers in urine samples

摘要

A simple,fast and sensitive capillary electrophoresis(CE) strategy combined with chemiluminescence(CL) detection for analysis of ofloxacin(OF) enantiomers was established in the present work.Sulfonated p-cyclodextrin(β-CD) was used as the chiral additive being added into the running buffer of luminol-diperiodatocuprate(Ⅲ)(K_[Cu(HIO_6)_2],DPC) chemiluminescence system.Under the optimum conditions,the proposed method was successfully applied to separation and analysis of OF enantiomers with the detection limits(S/N=3) of 8.0 nM and 7.0 nM for levofloxacin and dextrofloxacin,respectively.The linear ranges were both 0.010-100 μM.The method was utilized for analyzing OF in urine;the results obtained were satisfactory and recoveries were 89.5-110.8%,which demonstrated the reliability of this method.This approach can also be further extended to analyze different commercial OF medicines.