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Kinase and phosphatase activity measurement using fluorescence polarization
Kinase and phosphatase activity measurement using fluorescence polarization
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机译:使用荧光偏振测量激酶和磷酸酶活性
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摘要
A method for quantitating enzyme activity that causes phosphorylation or dephosphorylation of a peptide or protein substrate is provided. The method utilizes the differences in the fluorescence polarization of a phosphorylated amino acid-containing fluorescence-emitting reporter molecule free in solution as compared to being bound to a binding molecule.
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