Method for counting photons in laser-scanning systems

摘要

Method for photon counting in the detection channel of a laser scanning arrangement, preferably a laser scanning microscope, wherein the amplitude of the incoming photons is determined by means of a plurality of threshold values and the determination of thresholds is coupled with a time-resolved measurement in that pulse counting is carried out respectively for individual threshold values and the sum of the counted pulses is determined for the threshold values, wherein when a higher threshold value is reached, the counting value of the low threshold value is stored for the counting.