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Methods for identification and isolation of specific nucleotide sequences in cDNA and genomic DNA
Methods for identification and isolation of specific nucleotide sequences in cDNA and genomic DNA
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机译:鉴定和分离cDNA和基因组DNA中特定核苷酸序列的方法
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摘要
The present invention is directed to a method for the identification and isolation of specific genetic sequences from cDNA libraries or samples of genomic DNA derived from the cells, tissues or organs of an organism, particularly a mammal, and most particularly a human (including a human embryo or fetus). In particular, the invention is related to a method whereby a tissue-specific cDNA or a genetic marker, not found in cDNA libraries or genomic DNA samples derived from other tissues from the same or a different organism, may be identified and isolated using an Amplification Fragment Length Polymorphism (AFLP)-based technique. This method has applications in the identification and isolation of cDNA sequences and genomic genetic markers which may be used in a variety of medical, forensic, industrial and plant breeding procedures.
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