METHODS FOR IDENTIFICATION AND ISOLATION OF SPECIFIC NUCLEOTIDE SEQUENCES IN cDNA AND GENOMIC DNA

摘要

The present invention is directed to a method for the identification and isolation of specific genetic sequences from cDNA libraries or samples of genomic DNA derived from the cells, tissues or organs of an organism, particularly a mammal, and most particularly a human (including a human embryo or fetus). In particular, the invention is related to a method whereby a tissue-specific cDNA or a genetic marker, not found in cDNA libraries or genomic DNA samples derived from other tissues from the same or a different organism, may be identified and isolated using an Amplification Fragment Length Polymorphism (AFLP)-based technique. This method has application in the identification and isolation of cDNA sequences and genomic genetic markers which may be used in a variety of medical, forensic, industrial and plant breeding procedures. The figure is an autoradiogram of restriction fragments, separated by gel electrophoresis, of samples prepared from cDNA libraries of human liver, leukocytes, kidney and brain.