PRODUCTION OF HUMAN CNS NEURONS FROM EMBRYONAL CARCINOMA CELLS USING CELL AGGREGATION METHOD

摘要

The invention provides a method for rapid production of pure CNS neurons from a cancer cell line comprising the steps of: ;(a) replating an embryonic carcinoma cell line culture at a density of approximately 1×10⁵ cells per ml, and then allowing the culture to form cell aggregates by incubating the culture; ;(b) adding all-trans retinoic acid to the cell culture containing cell aggregates to obtain an all trans retinoic acid concentration ranging from about 1 to about 20 &mgr;m ;(c) incubating the culture until neurons are differentiated; and ;(d) isolating the neurons from the culture.