A METHOD FOR PREPARING MASSIVELY RmlC PROTEIN AND PURIFIED RmlC PROTEIN OF MYCOBACTERIUM TUBERCULOSIS BY IT

摘要

This invention relates to a mass preparation method of RmlC protein and the purified RmlC protein by the said method. RmlC is a gene product of rmlC, which is one of the biosynthesis genes of rhamnose that is an important element that consists cell wall of Mycobacterium tuberculosis. To be more specific, this invention improves the following disadvantage of recombinant RmlC protein of Mycobacterium tuberculosis previously reported that contains unnecessary 15 amino acids into the natural RmlC protein. This invention relates to a recombinant plasmid, a recombinant E. coli that is transformed by the plasmid, preparation method of Mycobacterium tuberculosis RmlC recombinant protein using the recombinant E. coli, purification method of recombinant RmlC protein, and the RmlC protein purified by the said method.