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Sorbitol dehydrogenase gene, a novel recombinant DNA, and a process for producing sorbitol dehydrogenase
Sorbitol dehydrogenase gene, a novel recombinant DNA, and a process for producing sorbitol dehydrogenase
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机译:山梨醇脱氢酶基因,新型重组DNA和山梨醇脱氢酶的生产方法
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摘要
A sorbitol dehydrogenase gene encoding (a) a protein having an amino acid sequence shown in SEQ ID NO: 1; or (b) a protein with sorbitol dehydrogenase activity consisting of an amino acid sequence comprising one or more deletions, substitutions or additions in the amino acid sequence of (a). Further, the invention provides a novel recombinant DNA having the sorbitol dehydrogenase gene inserted into a vector DNA, and a transformant or a transductant containing the recombinant DNA. Also, the invention provides a sorbitol dehydrogenase production method comprising the steps of: culturing the transformant or the transductant in a medium; and collecting sorbitol dehydrogenase from the culture. According to the present invention, sorbitol dehydrogenase can be produced efficiently.
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机译:一种山梨糖醇脱氢酶基因,其编码(a)具有SEQ ID NO:1所示氨基酸序列的蛋白质; (b)具有山梨糖醇脱氢酶活性的蛋白质,该蛋白质由在(a)的氨基酸序列中包含一个或多个缺失,取代或添加的氨基酸序列组成。此外,本发明提供了具有将山梨糖醇脱氢酶基因插入载体DNA中的新型重组DNA,以及含有该重组DNA的转化体或转导体。另外,本发明提供了一种山梨糖醇脱氢酶的生产方法,包括以下步骤:在培养基中培养转化体或转导体;并从培养物中收集山梨糖醇脱氢酶。根据本发明,可以有效地生产山梨糖醇脱氢酶。
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