Permeation cell for in vitro determination of skin permeation of pharmaceutical drugs

摘要

A permeation cell for the in vitro determination of the permeation of pharmaceutical active ingredients through the skin, having a donor chamber (34) and an acceptor chamber (4) that is separable therefrom, a window lying between them for receiving sealingly a skin membrane and sealingly closable filling and emptying openings at both chambers, characterized in that the acceptor chamber (4) is in the form of a cylindrical vessel that is upright during operation, having a closure (8; 50; 80) at its upper end and a side window opening (6) in the region near its base, and the donor chamber (34) is in the form of a connection piece that joins onto the acceptor chamber radially and can be closed at its outer end, with the exception of its closure (8; 50; 80) the acceptor chamber (4) is inserted removably into a housing (10) that fits closely around it, which housing has, in the region of the window opening (6) of the acceptor chamber, a corresponding slightly wider opening (30), and the connection piece forming the donor chamber (34) is screwed removably onto the housing concentrically with the window opening (6). As a result, the permeation cell gains stability, it is as easy to manipulate as it is to manufacture, auxiliary means for attaching the skin membrane not being required, and the acceptor chamber has only a small dead volume. By constructing the housing (10) essentially in block form, several such permeation cells can be used next to one another in a common heating and/or stirring device.