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Subtractive amplification kit useful in the diagnosis of genetic disease mutation or variation
Subtractive amplification kit useful in the diagnosis of genetic disease mutation or variation
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机译:减法扩增试剂盒可用于诊断遗传疾病的突变或变异
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摘要
A kit is provided for preferentially amplifying target RNA in a sample of tester RNA relative to non-target RNA in the sample, the kit driver sequences complementary to the non-target tester RNA under conditions where the driver sequences hybridize to the non-target RNA, a nucleic acid primer capable of hybridizing to the target RNA under conditions suitable for extension of the nucleic acid primer; and a promoter template capable of hybridizing to a DNA that is complementary to the target RNA under conditions suitable for extension of the complementary DNA such that a functional double promoter is formed.
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