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Fluorescence-based high throughput screening assays for protein kinases and phosphatases
Fluorescence-based high throughput screening assays for protein kinases and phosphatases
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机译:基于荧光的蛋白激酶和磷酸酶高通量筛选测定
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摘要
The invention relates to novel fluorescence-based assays for protein kinases and phosphatases which can be used in high throughput screening. The methods of the invention utilize a competitive immunoassay to determine the amount of substrate that is phosphorylated or dephosphorylated during the course of a kinase or phosphatase reaction to yield a product, as well as the phosphorylating or dephosphorylating activity of a kinase or phosphatase.
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