Cotton Transformation by Agrobacterium and medada Alte Efficiency using petiole explants

摘要

Is a method for producing a transgenic Cotton Plant through Agrobacterium mediated Transformation, the tissue of the petioles.The Method comprises the following steps: (a) obtain explants of Cotton petioles; (b) to expose the explants cultured petioles agrobacteriumtumefaciens which houses a Vector comprisingAn exogenous Gene and a selectable Marker, the Agrobacterium being able to carry out the transferenciaestable Gene and exogenous Gene of res Minor Agent SelectionTo the Genome of the cells of the petiole EXPLANT; (c) cultivate the explantespecu00edolo,So as to induce the formation of Callus; (d) - select the corns processed espresen the Exogenous Gene; (e) The calloseleccionado ar cultivated in suspension Culture for InducThe formation of embryoid; (f) - embriooides Regenerate whole plants in the form of algodontransgu00e9nicas.