A Gram negative bacterium origin comprising a mutation in a Gene, an immunogenic vaccine composition, a composition, a method for producing a Mutant of this bacterium, a method for producing such a polynucleotide attenuated bacteria, Purified and isolated the same, VEC

摘要

Origin is a Gram negative bacterium that has a mutation in a Gene represented by a sequence of nucleotides that do not appear in any of the. SEC.(Identification of Sequence Numbers): 1, 3, 7, 9, 21, 25, 27, 29, 39, 41, 51, 53, 55, 57, 58, 60, 68, 72, 74, 76, 78, 80, 82, 84, 104, 108, 112, 116, 118, 120, 122, 124, 126, 128 and 130, or their homologous species, producing such a mutation decreased activity in a Gene Product codif Qualified by the Gene mutated; also includes a mutation in a Gene, an immunogenic composition,A vaccine composition, a method for producing such a Mutant bacterium, a method for producing such a polynucleotide attenuated bacteria, Purified and isolated the same, a Vector, a host Cell, a method for producing a recombinant polypeptide, an Antibody, a method Using the monoclonal antibody and a method for identifying an antibacterial agent.Identify virulence genes of Gram negative bacteria, which allows the identification of New antibacterial agents that target these virulence genes and their products, and obtain New mutants useful in Gram negative bacterial Vaccines.