Recombinant viral vector, recombinant plasmu00ecdeo, kit, and methods to produce recombinant cells to detect the presence of vu00ecrus HIV in a sample.And to detect the presence of vu00ecrus HIV in a sample, and the resistance of HIV to drugs in a sample.

摘要

Recombinant vector, recombinant plasmu00ecdeo, kit, and methods to produce recombinant cells to detect the presence of vu00ecrus HIV in a sample.And to detect the presence of vu00ecrus HIV in a sample, and the resistance of HIV to drugs in a sample.There are provided methods and recombinant expression vectors for the detection of HIV and to monitor the drug resistance against HIV.The method comprises the steps of: obtaining a cell culture; add a recombinant viral vector for transduzir in culture cells.Recombinant viral vector comprising a reporter gene sequ00b3u00eancia comprising a reporter whose expression is regulated by a protein specific for the HIV virus that is expressed in a genome of a Vu00edrus HIV during the infection of a cell in a culture that is transduzida by recombinant viral vector, and a sequ00b3u00eancia receiver comprising a CD4 gene and one or more genes co receptor.The expression of genes co receptor by facilitating the productive infection of the cell transduzida and allowing the HIV virus that infected the cell transduzida if replicate and infect cells it infectsIn cell culture transduzidas by recombinant viral vector; infect cells transduzidas with a sample containing HIV; adding one or more anti HIV agents in cell culture and stop;Ctar a change in a level of expression of the reporter gene in cells.