The cell of yeast and detection methods of interaction between a first peptu00ecdio with a second peptu00ecdio a pair of connection of peptu00ecdios,Determination of interaction of a test specimen with a first or a second peptu00ecdios a pair of connection of peptu00ecdios and simultaneous detection of the interaction of two pairs of connecting peptu00ecdTwo different

摘要

The "cell of yeast and detection methods of interaction between a first peptu00ecdio with a second peptu00ecdio a pair of connection of peptu00ecdios,Determination of interaction of a test specimen with a first or a second peptu00ecdios a pair of connection of peptu00ecdios and simultaneous detection of the interaction of two pairs of connecting peptu00ecdTwo different ".This invention relates to new host cells that express heterologous proteins modified rendered and screening methods of test specimens with binding activity of peptides in the Cu00e9lula stewardess modified comprises: (a) a sequ00b3u00eancia genes coding for a heterologous fusion protein.This fusion protein comprising a first peptide of a pair of binding peptides or segment of the first peptide which is added to a DNA binding domain.To its corresponding domain transcriptional activation of a protein for transcriptional activation; (b) a sequ00b3u00eancia genes coding for a heterologous fusion protein.This fusion protein comprising a peptide of the second pair of binding peptides (a) or a segment thereof, fused to a DNA binding domain.To its corresponding domain transcriptional activation.None of which is used in (a); (c) a gene operatively linked to luciferase protein transcriptional activation or part thereof; (d) optionally.A deletion or change in the chromosomal DNA of the host cell to the protein of transcriptional activation, if present in the host cell selected.