Using nuclear magnetic resonance sup 13 c to detect linkage disequilibrium to target molecules

摘要

A method for Detecting linkage between a ligand and a putative pre selected Target Molecule with 13C enriched,Method comprising: (a) generating a First Correlation Spectrum of two-dimensional 1h / 13C NMR of the target Molecule; (b) forming a mixture of the target Molecule with at leastA putative ligand compound; (c) a Second Generation of 13C / 1H NMR Spectrum of two-dimensional correlation of the mixture from step (b); and (d) a comparison of the first and second Spectra.